ABSTRACT
Objective Toexploretheclinicalvalueofcombiningapplicationofthreedimensionalpseudocontinuousarterialspin labeling (3D-pCASL)andsusceptibilityweightedimaging (SWI)inpredictingtheoutcomsofcerebralischemia.Methods Thirty-two patientswithmoderatetoseverestenosisofunilateralmiddlecerebralartery(MCA)and16healthyvolunteers(asacontrolgroup) underwenttheexaminationsoftheroutineMRI,MRA,3D-pCASL (PLD:1525ms,2525ms)andSWI.Thepatientsweredividedinto twogroups:lowperfusiongroupandequal/highperfusiongroupaccordingtotherCBFof3D-pCASLwithPLD:2525ms.OnSWI, theparametersofdeepmedullaryvein(DMV)includedratioofquantity,totallengthinbilateralunitareaandsubjectivescoreofischemic side.ThedifferencesofthevaluesofDMV parametersamongthegroupswerecompared,andthecorrelationsbetweenrCBFand DMVparameterswereanalyzed.Thereexaminationswereperformed6monthslaterandtheincidenceofinfarctionwascomparedbetween thelowperfusiongroupandtheequal/highperfusiongroup.ThecorrelationbetweenvaluechangeofrCBFandDMV,andthetransformation tendencyfromtheischemiclesiontocerebralinfarctionwerealsoanalyzed.Results Inthelowperfusiongroup,allDMVparameters werehigherthanthoseinthecontrolgroupandtheequal/highperfusiongroup,andthedifferencesbetweengroupswerestatistically significant(P＜0.05),buttherewerenosignificantdifferencesbetweenthevaluesofthecontrolgroupandtheequal/highperfusion group (P＞0.05).Thereexaminationresultsof6monthslatershowedthat4caseswithcerebralinfarctionwereallfromthelowperfusion group.Theincidenceofthecerebralinfarctioninthelowperfusion groupwas25%andwassignificantlyhigherthanthat(0%)in theequal/highperfusiongroup (P＜0.05).Theincidencewas positivelycorrelatedwiththeratioofquantity,totallengthinbilateral unitareaandsubjectivescoreofischemicsideofDMV (r＝0.512,0.43,0.449,P＜0.05),whilenegativelycorrelatedwithrCBF (r＝－0.501,P＜0.05).Conclusion TherearesomechangesofrCBFandDMVincerebralischemicarea,andthereisacorrelationbetween rCBFandDMV.3D-pCASLandSWIcanshowcerebralbloodflowperfusionandDMVinischemicarea,andplayanimportantrolein studyingtheoutcomesofcerebralischemiclesions.
ABSTRACT
Objective To investigate the relationship between the expression patterns of fibronectin (Fn) and its receptors-α5β1 and αVβ3 integrins and angiogenesis after cerebral ischemia in aged mice. Methods Both young (2 months) and aged (18 months) C57BL6 male mice were subject to middle cerebral artery occlusion. The brain endothelial cells (BECs) proliferation was assessed by using dual-immunofluorescent staning (IF) for Ki67 and CD31 and the vessel density was quantified by counting the number of CD31-positive vessels per field of view. The expression patterns of Fn,α5β1 and αVβ3 on blood vessels was assessed by using dual-IF for CD31/ɑ5,CD31/β3 and CD31/Fn. Results The number of CD31/Ki67 dual-positive cells in the penumbra at day 7 after cerebral ischemia of was significantly lower in the aged mice than in the young mice (4.7±0.8/field, P<0.05). The number of CD31-positive vessels in the penumbra at day 7 and 14 of was both significantly lower in aged mice than in the young mice (38.3±3.9/field, 45±4.4/field, both P<0.01). Furthermore, the expression of Fn, α5 and β3 on blood vessels in the penumbra at day 7 after cerebral ischemia was significantly lower in age mice than in the young mice (P<0.05). Conclusions The cerebral angiogenesis after ce?rebral ischemia is impaired in the aged stroked mice, which is associated with the low expression of fibronectin and its receptors-α5β1 andαVβ3 integrins on blood vessels.
ABSTRACT
Objective To investigate the metabolic alterations in the brain of neonates with HIE and correlate those alterations with clinical grading and prognosis of HIE.Methods Fourty-six eases of full-term neonates diagnosed as HIE clinically were performed MRI and 1~H-MRS,9 healthy neonates without the evidence of asphyxia were studied as controls,1~H-MRS techniques included single voxel proton MRS and two dimensional muhi-voxel chemical shift spectroscopy imaging,point resolved spectroscopy sequence was used for 1~H-MRS.Metabolic changes in the spectroscopy were analyzed in neonates with HIE,and study the relationgship between MRS findings and prognosis.Results(1)The typical 1~H-MRS manifestations of full- term neonates suffering from HIE were as follows:the peaks of Lac were elevated,GLx-? were elevated and NAA were decreased.(2)GLx-?/Cr ratio in control,mild,moderate and severe HIE group was 0.16, 0.21,0.64,and 1.31,respectively.Lac/Cr ratio in control,mild,moderate and severe HIE group was 0.12,0.14,0.19,and 0.26,respectively.There was a significant difference in the ratio of GLx-? and Lac/Cr between HIE group and control group(t=5.01,P